Droplet Microfluidics for 3D Epithelial Cell Culture
Poster Mar 25, 2014
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan
We have developed a microfluidic system which delivers highly-controlled conditions for the seeding and growth of polarized cellular spheres, or acini, within matrigel beads. We use high-throughput flow handling to change culture conditions and to move acini through flow-analysis devices on an acini-by-acini basis. Using these optimized parameters we can now design RNAi screens that will identify genes important for the regulation of acini formation in a 3D environment.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.