Enhancement of proliferation in a novel rat hepatocyte co-culture model after mitogenic stimulation
Poster Mar 13, 2012
Susan Hester, Michael A. McVay, Oyinade Adefuye, Alan Tennant, and Salman Khetani
Primary mouse and rat hepatocyte cultures have long been the gold standard for assessment of cellular changes associated with chemical exposure. While helpful for assessing proliferative and apoptotic responses in vitro, these cultures have limitations due to loss of cells beyond 48hrs incubation. Rat HepatoPac™ was used to extend the culture time beyond 3 days and coupled with downstream quantitative high content imaging (HCI) technology for a more accurate assessment of the cell proliferative response following mitogenic stimulation.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE