Enhancement of proliferation in a novel rat hepatocyte co-culture model after mitogenic stimulation
Poster Mar 13, 2012
Susan Hester, Michael A. McVay, Oyinade Adefuye, Alan Tennant, and Salman Khetani
Primary mouse and rat hepatocyte cultures have long been the gold standard for assessment of cellular changes associated with chemical exposure. While helpful for assessing proliferative and apoptotic responses in vitro, these cultures have limitations due to loss of cells beyond 48hrs incubation. Rat HepatoPac™ was used to extend the culture time beyond 3 days and coupled with downstream quantitative high content imaging (HCI) technology for a more accurate assessment of the cell proliferative response following mitogenic stimulation.
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE