Enzyme Coated Magnetic Nanoparticles For Use In Continous Flow Microfluidic Devices
Poster Mar 17, 2015

Ferenc Ender, Diana Weiser, Andras Vitez, Laszlo Poppe
Magnetic nanoparticles (MNPs) are widely used as bio molecule carriers providing higher reactive area and low diffusion limits, specific modifications enabling convenient handling within chip sized fluidic structures. Our infrastructure for the production of tailor made MNPs enables to optimize the particles for the use in a LoC platform. The novel platform incorporates micro-sized magnetic reaction cells capable of anchoring protein-coated MNPs addressable and selectively. Such platform allows the design of highly flexible and "programmable" execution of multienzyme processes for biocatalytic or diagnostic purposes.
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When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.
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