Enzyme Coated Magnetic Nanoparticles For Use In Continous Flow Microfluidic Devices
Poster Mar 17, 2015
Ferenc Ender, Diana Weiser, Andras Vitez, Laszlo Poppe
Magnetic nanoparticles (MNPs) are widely used as bio molecule carriers providing higher reactive area and low diffusion limits, specific modifications enabling convenient handling within chip sized fluidic structures. Our infrastructure for the production of tailor made MNPs enables to optimize the particles for the use in a LoC platform. The novel platform incorporates micro-sized magnetic reaction cells capable of anchoring protein-coated MNPs addressable and selectively. Such platform allows the design of highly flexible and "programmable" execution of multienzyme processes for biocatalytic or diagnostic purposes.
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE