EU-OPENSCREEN - The European Research Infrastructure of Open Screening Platforms for Chemical Biology
Poster Feb 20, 2015
EU-OPENSCREEN (www.eu-openscreen.eu) is the largest emerging academic chemical biology research infrastructure initiative in Europe with the aim to collaboratively develop novel research tool compounds with external scientists. As a joint effort of national networks in 16 European countries, EU-OPENSCREEN offers access to high-throughput screening platforms, chemistry services and a large compound collection. It invites biologists who have a robust and suitable biological assay to collaboratively develop chemical tool compounds to validate their targets-of-interest. Selected assays are screened against a collection of 100,000-200,000 compounds, incl. confirmatory and counter screening, IC/EC50 and SAR determination, and QC of confirmed hit compounds. Chemists are invited to provide compounds to be included into the jointly used EU-OPENSCREEN compound collection, which is screened against a wide range of biological assays, thereby delivering extensive information about the biological activities of their compounds. EU-OPENSCREEN adopts an open-access policy to support maximal data dissemination and publication.
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE