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Evaluation of an automated SPE method for samples derived from capillary microsampling
Poster

Evaluation of an automated SPE method for samples derived from capillary microsampling

Evaluation of an automated SPE method for samples derived from capillary microsampling
Poster

Evaluation of an automated SPE method for samples derived from capillary microsampling

• The automated workflow described gave equivalent results (LLOQ, precision, accuracy) compared to samples processed manually by protein precipitation. The overall time/sample is slightly higher for the automated protocol. However, since only minimal intervention is required, process safety is superior and human resources are freed up for other tasks.

• The automated protocol may be further optimized in several aspects. As shown a volume of 20 μL is sufficient for complete elution of the analyte from the cartridge. Figure 6 indicates that a loading volume of 2-5 μL diluted plasma is sufficient allowing the use of low-volume samples. Both aspects are important for further optimizing this protocol for analysis of samples originating from studies using microsampling techniques.

• Methods for the automated optimization of the SPE conditions exist and will be applied in future studies.
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