Evaluation of Different Interpretation Strategies to Discover PTM in MS/MS Peptide Fragmentation Data
Poster Apr 05, 2007
Daniel Chamrad, Gerhard Korting, Ken Fantom, Andy West, Klaus Schneider, Ulrike Schweiger-Hufagel, Herbert Thiele and Martin Bluggel
The phenomenon of acquired high quality MS/MS spectra that can not be explained within typical sequence database searches is well known. Although protein identification was successful it is manually very laborious and in most cases even impossible to match these spectra with any suggested protein sequence.
The procedure of second pass searches has been developed to overcome this problem. Here we report from our in house developed tool PTM-Explorer (Protagen AG, Dortmund, Germany; Bruker Daltonik GmbH, Bermen, Germany). Two LC-MS/MS datasets of recombinant kinase proteins (MAPK 12 and MAPK 13) have been used for the evaluation.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE