Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA
Poster Aug 19, 2016
Hidevaldo B. Machado, John A. Schiel, Maren Mayer-Gross, Eldon T. Chou, Melissa L. Kelley, Anja van Brabant Smith. Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
The bacterial CRISPR-Cas9 system has revolutionized the genome engineering world with its efficiency and ease of use. The most common use of the CRISPR-Cas9 technology has been to engender gene knockouts that are generated as a result of imperfect repair of a targeted double-strand DNA break by the non-homologous end joining (NHEJ) pathway.
Identification of genes involved in cell cycle regulation using arrayed synthetic CRISPR RNA libraries in a multiparameter high-content assayPoster
Gene knockout using CRISPR-Cas9 has dramatically transformed biological research and has been rapidly applied to loss-of-function screening primarily using pooled lentiviral sgRNA libraries. A synthetic CRISPR RNA (crRNA) approach is amenable to screening in arrayed, well-by-well fashion and expands the types of phenotypic readouts that can be used, including high-content and morphology-based assays.READ MORE
Nitrogen metabolism in Mycobacterium tuberculosis: a systems-based approachPoster
Previous studies suggested that Mycobacterium tuberculosis obtains nitrogen from a diverse range of intracellular nutrients including amino acids. Here, we use a novel system’s based three-pronged approach to define pathways for uptake and assimilation of nitrogen.READ MORE
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
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