Exploring The Benefits Of Automated Unattended Sample Derivatization Prior To Gas Chromatography Analysis
Poster Jun 10, 2014
A. Caruso, M. Santoro, P. Magni, S. Pelagatti, R. Facchetti
Sample derivatization is often used in GC and GC/MS analyses, to enhance the volatility of certain compounds.
Strong intermolecular attractions between polar groups such as NH, SH or OH, groups can create hydrogen bonds that lead to molecular attraction and reduce volatility. Replacing hydrogen in these groups by alkylation, acylation, or silylation significantly increases volatility, especially in compounds with multiple polar groups. Typically, the replacement of active hydrogens with trimethylsilyl groups leads to volatile derivatives that can readily undergo GC analysis.
A typical silylation reaction requires the addition of a reagent such as MSTFA, TMCS, or BSTFA in presence of a base in an anhydrous and heated environment and may last several minutes. The length of the reaction, and the fact that often these additions of liquid reagents must be carried out manually are downsides of the process and negatively affect overall laboratory throughput.
The introduction of a system capable of handling much of the sample preparation process automatically can increase laboratory throughput and free the operator from the most tedious, time-consuming and error-prone steps.
The Thermo Scientific™ TriPlus™ RSH autosampler is an example of such a system.
The TriPlus RSH autosampler is a versatile automatic sampling platform that is also capable of advanced sample preparation cycles, enabling excellent precision and reproducibility and ensuring unattended and reliable operations.
Using Elemental Analysis For Discrimination Of Pinot Noir Wines From Six Different Districts In An AvaPoster
The determination of geographical origin of wine is gaining increased interest by researchers and federal agencies around the world, partially due to increased fraud with regards to place of origin labelling. For wine, multi-elemental profiling of macro, micro, and trace elements has been proposed for determination of authenticity. Commercial wines from different wineries in 5 different neighborhoods within one AVA show characteristic elemental fingerprints. Macro, micro and trace elements as well as elemental ratios contribute to the observed separation, indicating the involvement of multiple factors and underlying mechanisms, including location and soil composition, elemental uptake by vine and rootstock, viticulture and nutrient management, water sources, and small differences in the different wineries.READ MORE
Fast arsenic speciation analysis of wines and rice with LC-ICP-QQQPoster
This method was designed in response to recent and proposed food standards, both international and national, that limit inorganic arsenic rather than total, organic, or individual arsenic species such as arsenite (AsIII) and arsenate (AsV). Analysis time is 10x faster than the current FDA regulatory method, increasing sample throughput, avoided spectral interferences and dramatically increased sensitivity. Validation data from two laboratories demonstrate the method’s accuracy and reproducibility of both wine and rice matrices in a single analytical batch.READ MORE
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.