Exploring the Therapeutic Potential of a Peptide Derived from a Poxviral Immune Evasion Protein
Poster Aug 04, 2014
Toll-like receptors (TLRs) have a role in viral detection leading to cytokine and IFN induction, and as such are targeted by viruses for immune evasion.
The poxviral protein A46 has been identified to inhibit TLR signalling by interacting with TIR domain containing proteins of the receptor complex to
collectively inhibit all TLR adaptor proteins that positively regulate transcription-factor activation. An inhibitory peptide derived from A46 termed VIPER
selectively targets TLR4. This research investigates what affect the polyarginine delivery sequence location has on VIPER activity in vitro. In this study,
9R-VIPER is shown to be more effective at TLR4 and TLR2 signal inhibition than VIPER for multiple signalling pathways. Novel mechanisms of TLR2-
driven interferon induction have been shown to be affected by 9R-VIPER and VIPER. The longevity of TLR4 inhibition by 9R-VIPER was shown to be
superior to VIPER. Residues required for peptide activity were identified. Structural information about 9R-VIPER and a loss-of-function mutant was
determined by NMR analysis.
Designing a Model to Explore Tau's Unfolded Protein ResponsePoster
The purpose of this research is to design a cell model in which ER stress caused by tau accumulation can be generated, and then investigated for changes in different ER stress-associated proteins.READ MORE
A New Method for Analyzing MSe/All Ions Fragmentation in Xenobiotic Metabolism StudiesPoster
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE
Extracorporeal shockwave therapy accelerates motor axon regeneration despite a phenotypically mismatched environmentPoster
A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.READ MORE