Exploring the Therapeutic Potential of a Peptide Derived from a Poxviral Immune Evasion Protein
Poster Aug 04, 2014
Toll-like receptors (TLRs) have a role in viral detection leading to cytokine and IFN induction, and as such are targeted by viruses for immune evasion.
The poxviral protein A46 has been identified to inhibit TLR signalling by interacting with TIR domain containing proteins of the receptor complex to
collectively inhibit all TLR adaptor proteins that positively regulate transcription-factor activation. An inhibitory peptide derived from A46 termed VIPER
selectively targets TLR4. This research investigates what affect the polyarginine delivery sequence location has on VIPER activity in vitro. In this study,
9R-VIPER is shown to be more effective at TLR4 and TLR2 signal inhibition than VIPER for multiple signalling pathways. Novel mechanisms of TLR2-
driven interferon induction have been shown to be affected by 9R-VIPER and VIPER. The longevity of TLR4 inhibition by 9R-VIPER was shown to be
superior to VIPER. Residues required for peptide activity were identified. Structural information about 9R-VIPER and a loss-of-function mutant was
determined by NMR analysis.
Human iPSC-derived cardiomyocytes: A translational model to predict drug-induced cardiac arrhythmias and long-term toxicityPoster
The results shown here imply that iPSC-derived Cor.4U human cardioymocytes are a translational in vitro cell model for the prediction of clinically relevant drug-induced cardiac arrhythmias and long-term toxicity.READ MORE
Fc Effector Bioassays for Rapid and Quantitative Measurement of ADCC and ADCP Mechanisms of ActionPoster
Drug developers are rapidly adopting Fc effector function reporter-based bioassays to measure antibody Fc functions such as ADCC and ADCP activity during the development of therapeutic antibodies. Here we show application of a suite of FcgR Reporter Bioassays to elucidate and characterize antibody MOA.READ MORE
Quantitative Live-Cell Analysis Using Automated Long-Term ImagingPoster
Here we describe a continuous live-cell assay for determining cell proliferation profiles using the BioSpa Automated Live Cell Imaging System, consisting of BioSpa 8 and Cytation 5.READ MORE