Extract Optimum Value from Screening data using a Modern Complete Screening Solution
Poster Apr 25, 2007
Laurence Painell and Glyn Williams
Modern screening technologies are becoming more and more complex; the magnitude of data produced is much greater than previous generation techniques. With more volume of data comes increased complexity with the handling, analysis and management of the data. Much of the data needs to be analyzed in discrete chunks using different techniques, these independent analyses then need to be tied together to create a complete view of the results. It is important that any system employed is capable of scaling up with the data analysis and management needs of the organization.
There are many new technologies on the market that are capable of producing large volumes of data using modern techniques, the problem then lies in the analysis; storing the results along with the criteria under which it was captured, including all the contextual data.
With the use of numerous standardized biochemical assays now commonplace, successful application of screening technologies requires accurate and meaningful handling of the experimental results. Specifically, this means capturing the vast data volumes generated and subsequent validation and verification. Decision making can be supported via comprehensive and intuitive visualization of results. Also important is the establishment and maintenance of analysis templates for this type of experiment.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE