Facing the challenges in bio-pharmaceutical production: newly developed polymer-based ion exchange chromatography media and their application to the purification for Immunoglobulin – from egg yolk
Poster Jun 03, 2011
Masakatsu Omote, Noriko Shoji, Naohiro Kuriyama and Daniel Kune
However, with the progress made in recent years, titers in fermentation processes have increased significantly. Obviously, this increased volumetric productivity will help reducing the cost of goods, but it also has an impact on the downstream processing. This means that improved downstream processing media are required to handle the increased product load in the same timeframe.
YMC has recently developed several polymer-based IEX media, known as YMC-BioPro Q and S (with quaternary ammonium [QA] and sulfobutyl [SB] functional groups, respectively). YMC-BioPro IEX media are based on the same porous hydrophilic polymer beads with 30 or 75 μm diameters, modified with the respective surface ligands. This new combination of matrix and surface ligand provides higher binding capacity and higher recovery for bio-molecules compared to conventional IEX media. The spherical beads, with optimal surface modification, provide high dynamic binding capacities (DBC) even at high flow rates up to 1000 cm/h. Excellent selectivity is achieved by bonding QA and SB functional groups to the ligand. Both factors impact on process economy, as high flows will allow fast separations whilst excellent selectivity will increase purity.
We show benefits of YMC-BioPro materials for superior downstream processing and separation of important bio-molecules, such as monoclonal antibodies and Immunoglobulin Y (IgY) from egg yolk.
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