Fast arsenic speciation analysis of wines and rice with LC-ICP-QQQ
Poster Dec 05, 2017
Patrick Gray, Courtney Tanabe, Susan E. Ebeler, Jenny Nelson.
This fit-for-purpose method was designed in response to recent and proposed food standards, both international and national, that limit inorganic arsenic rather than total, organic, or individual arsenic species such as arsenite (AsIII) and arsenate (AsV). In this method, AsIII is intentionally oxidized to AsV with H2O2 during sample preparation, converting all inorganic arsenic (the sum of AsIII and AsV) to the AsV form. Arsenic species were separated in less than 2 minutes using a short, narrow bore, 5 um chromatography column. This analysis time is 10x faster than the current FDA regulatory method. The use of O2 reaction gas with inductively coupled plasma triple quadrupole mass spectrometry (ICP-QQQ with MS/MS capability) avoided spectral interferences and dramatically increased sensitivity, allowing for low volume injections. The small injection volume and modified mobile phase composition mitigate non-spectral interferences such as carbon enhanced ionization. Furthermore, the shortened analysis time significantly increases sample throughput. Validation data from two laboratories demonstrate the method’s accuracy and reproducibility of both wine and rice matrices in a single analytical batch.
Results are presented that demonstrate the accuracy and reproducibility of the new method. The method was further validated using a wine matrix that was analyzed by two participating laboratories.
The method presented here has been shown to work for both wine and rice matrices. Runtime has been decreased to 2 minutes, which is a factor of 10x faster than previous methods. The method also showed improved sensitivity and limits of detection and quantification compared to the current FDA method. Small injection volumes and addition of methanol in the mobile phase increased robustness to non-spectral interferences and makes this method more suitable for wine speciation. If needed, analysis time and resolution could be further improved by decreasing injection volume, increasing mobile phase flow rate and using a small volume,fast-washout spray chamber.
A New Method for Analyzing MSe/All Ions Fragmentation in Xenobiotic Metabolism StudiesPoster
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE
Using Elemental Analysis For Discrimination Of Pinot Noir Wines From Six Different Districts In An AvaPoster
The determination of geographical origin of wine is gaining increased interest by researchers and federal agencies around the world, partially due to increased fraud with regards to place of origin labelling. For wine, multi-elemental profiling of macro, micro, and trace elements has been proposed for determination of authenticity. Commercial wines from different wineries in 5 different neighborhoods within one AVA show characteristic elemental fingerprints. Macro, micro and trace elements as well as elemental ratios contribute to the observed separation, indicating the involvement of multiple factors and underlying mechanisms, including location and soil composition, elemental uptake by vine and rootstock, viticulture and nutrient management, water sources, and small differences in the different wineries.READ MORE
Elemental profiles of whiskies allow differentiation by type and region by inductively coupled plasma –optical emission spectroscopy (ICP-OES)Poster
The analysis of the elemental composition of whiskies provides a host of important information including sample origin and understanding how different whiskey styles are caused by processing equipment and raw materials. Preliminary data analysis of the whiskies showed that element compositions could possibly be used to differentiate samples based on age, type, and region.READ MORE
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World Advanced Therapies & Regenerative Medicine Congress
May 16 - May 18, 2018