Fast Liquid Differential Scanning Calorimetry (FLDSC)
Poster Apr 23, 2013
R. Splinter, A.W. van Herwaarden, A. Pfreundt, W.E. Svendsen, D. Istrate, W. van Eijk
Many types of cancer have been distinguished by measuring the thermal denaturation of proteins in blood plasma of patients. While conventional Differential Scanning Calorimetry (DSC) carries out such measurements with scan rates of 1 °C/min, requiring relatively large amounts of sample, Fast Differential Scanning Calorimetry (FDSC) operates at much higher scan rates of up to 1000 °C/s. Besides reducing the measurement time from hours to minutes, this lowers the required sample amount from hundreds to just a single (l. This poster introduces the new Fast Liquid Differential Scanning Calorimetry (FLDSC) sensor, showing experimental results on lysozyme protein solutions at various fast scanning rates.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE