Fingerprinting analytical strategy used for authentication of honey
Poster Nov 05, 2013
Oana Romina DINCAa,b, Roxana Elena IONETEa*, Raluca POPESCUa, Radu TAMAIANa, Diana COSTINELa, Gabriel Lucian RADUb
To find the most reliable methods for authentication of food or food ingredients has always been a challenging issue. Our research is of particular interest in the geographical discrimination of honey from different floral sources and different regions of Romania as support for quality assessment and classification activities. This study demonstrates both the benefits and the advantages of coupling different analytical techiques in order to ensure honey authenticity due to the fact that honey is a complex mixtures of molecules (toxins and peptides) and a challenging product to analyze. The control of quality and the assumption of the claimed botanical and geographical origin of honey, which is associated to the producing vegetation area, flowering period of the plants, are of prime importance for stakeholders from apiary industry in order to reinforce the consumer trust. Concerning the various possibilities of honey adulteration, peptide mass fingerprinting (PMF) and protein profiling methods have been used. This study reports also the use of isotope ratio mass spectrometry (IRMS) and site-specific natural isotopic fractionation measured by nuclear magnetic resonance (SNIF-NMR) for the assignment of origin and the proof of adulteration. The analyzed honey samples were from different geographical areas and different botanical origin. A fast, suitable and alternative technique to classify honey samples according to their geographical origin was developed based on PMF using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Acknowledgement: This study has been financed by the Romanian Ministry of Education and Research, National Authority for Scientific Research, 19N/2009 NUCLEU Program, under Project PN 09190205: „Investigations concerning natural products' characterization and authentication based on their protein profile assessment”, and with the support of the doctoral School “Faculty of Applied Chemistry and Materials Science” Politehnica University of Bucharest.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE