First total synthesis of Combretastatin D-2 congeners named Isocorniculatolide A and 11-o-methylisocornicultolide
Poster Jun 07, 2012
Vijaya Kumar.Tulam, Subhash Chandra Bose. Kotte, Sanjay Kumar. Hirasker, Prathama S Mainkar, P.M. Murali and K. Mukkanti.
First total synthesis of newly isolated natural products Isocorniculatolide A and 11-o-methylisocornicultolide A was accomplished. Commercially available 3, 4 dihydroxy benzaldehyde was selectively protected at the 4- position followed by the Wittig elongation to afford α, β unsaturated ester which was subsequently treated with DIBAL to afford (E)-2-(benzyloxy)-5-(3-hydroxyprop-1-enyl) phenol and then finally coupled this intermediate with 3-(4-bromophenyl) propanoic acid to achieve (Z)-3-(4-(benzyloxy)-3-hydroxyphenyl) allyl 3-(4-bromophenyl) propanoate. Finally macro cyclization using Ullmann ether synthesis and then treatment with Pd/C accomplished Isocorculatolide A. In the same way we achieved 11-o-methylisocornicultolide starting from Isovanillin.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.