First total synthesis of Combretastatin D-2 congeners named Isocorniculatolide A and 11-o-methylisocornicultolide
Poster Jun 07, 2012
Vijaya Kumar.Tulam, Subhash Chandra Bose. Kotte, Sanjay Kumar. Hirasker, Prathama S Mainkar, P.M. Murali and K. Mukkanti.
First total synthesis of newly isolated natural products Isocorniculatolide A and 11-o-methylisocornicultolide A was accomplished. Commercially available 3, 4 dihydroxy benzaldehyde was selectively protected at the 4- position followed by the Wittig elongation to afford α, β unsaturated ester which was subsequently treated with DIBAL to afford (E)-2-(benzyloxy)-5-(3-hydroxyprop-1-enyl) phenol and then finally coupled this intermediate with 3-(4-bromophenyl) propanoic acid to achieve (Z)-3-(4-(benzyloxy)-3-hydroxyphenyl) allyl 3-(4-bromophenyl) propanoate. Finally macro cyclization using Ullmann ether synthesis and then treatment with Pd/C accomplished Isocorculatolide A. In the same way we achieved 11-o-methylisocornicultolide starting from Isovanillin.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE