Poster May 05, 2011
Chris de Graaf, Gerdien de Kloe, Henry Vischer, Mark Verheij, Saskia Nijmeijer, Azra Delic, David Maussang, Ken Chow, Anitha Shanmugham, Paul England, Rogier Smits, Rob Leurs and Iwan de Esch
The poster demonstrates the following:
• All screens have resulted in unique and novel fragment hits.
• The use of fragments in chemogenomics approaches results in higher resolution interaction fingerprints and leads to improved structural understanding and novel insights in ligand binding characteristics.
• These studies can support the design of novel ligands with specified activity profiles.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE