Fragmentor: A New Technique to Facilitate Scaffold Design for Library Generation
Poster Oct 17, 2012
Sheppard D, Macritchie J & Allen V
To ensure that BioFocus’ libraries are at the cutting edge of innovation, they continually aim to evolve and improve our processes for design which can often instigate the generation of novel tools or methods that underpin our concepts for new target classes. Fragmentor is one such tool.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE