Function of Protein Kinase C-1 in memory in Caenorhabditis elegans
Poster Jun 12, 2013
Neil Patel, Shivon Manchanda, Aarohi Shah, Melissa McSweeney, and Aryeh Routtenberg
Assays that utilized their naïve attractive response were performed on N2 wild-type C. elegans and the pkc-1 mutant nj1, to determine how long the effects of the associative conditioning to these odors would last. We compared chemotaxis indices following conditioning for delay periods of 30 min, 4 hours, and 12 hours. We found that wild-type C. elegans form food-odor associations and retain them for at least 12 hours. Results show that nj1 displays less of a reduction in affinity following conditioning as compared to wild-type, which demonstrates an impairment in memory. Our results also showed a decreased affinity towards benzaldehyde in naïve nj1 nematodes, suggesting an impairment in the olfactory pathway. Thus, nj1 show not only impairments in food-odor associative memory, but potentially impairments in the olfactory pathway as well.