GC-MS/MS ANALYSIS OF BENZODIAZEPINES USING ANALYTE PROTECTANTS
Poster Jun 10, 2014
Jeremy Matthews, Alex Chen, Flavio Bedini
Benzodiazepines drugs are psychoactives whose structure contains a benzene ring fused with a diazepine ring. One of the most well known benzodiazepines – diazepam – has been marketed under the name “Valium” since 1963. These drugs are effective tranquilizers and as such are commonly used in medication to treat anxiety and sleep disorders amongst other conditions. The relative availability of these drugs combined with their sedative effect has led to their illicit use as either recreational drug and sometimes in suicides. Consequently it is common practice to analyze for benzodiazepines in forensic and toxicology laboratories.
Benzodiazepines compounds are bases and so readily react with active sites in eg. the GC inlet liner causing problems in analysis at low levels and resulting in poor linearity and reproducibility. The use of analyte protectants reduces liner activity and often enables the detection of such ‘active’ compounds at much lower levels. The use of analyte protectants in the GCMS analysis of a group of benzodiazepines was investigated and the results are discussed below.
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
C1 Complement mediates human cord blood serum derived APP α-secretase cleavage activity in vitroPoster
Our results indicate that CBS contains proteins that promote α-secretase like enzymatic activity. LC-MS/MS analysis in CBSF and AgBSF revealed the presence of 142 proteins of which C1 subunits and alpha-2-macroglobulin showed significantly greater levels in αCBSF compared with αAgBSF. further study showed,C1 subunits can enhance sAPPα production and Aβ reduction in cell culture conditionREAD MORE