Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA
Poster Oct 04, 2012
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi
Recently, there has been significant interest in the use of mRNA based expression systems for gene therapy applications and for the generation and manipulation of stem cells. Several groups have shown that mRNAs are attractive vehicles for therapeutic gene expression in mammals (Kormann et. al. Nat. Biotechnol (2011) 29, 154; Kariko et. al. Molecular Therapy (2012) Epub ahead of print). Additionally, Warren et al. demonstrated highly efficient induced pluripotent stem cell (iPSCs) generation by transfection of mRNAs encoding reprogramming factors (Warren et al. Cell Stem Cell (2010) 7, 618). The authors suggested that iPSCs generated in this manner should be safer than iPSCs derived by plasmid transfection or viral transduction as there is no risk of insertional mutagenesis and subsequent oncogenesis.
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
An Emerging Phenotype of Partial RAG 1/2 Deficiency Among Young Children with Autoimmunity and Viral InfectionsPoster
We describe the natural history of a cohort of 12 patients with confirmed partial RAG1/2 mutations and autoimmunity at a young age. We were seeking the link between viral infections and autoimmunity and tested candidate biomarkers that may reflect the underlying RAG1/2 protein deficiency.READ MORE
Quantitative Live-Cell Analysis Using Automated Long-Term ImagingPoster
Here we describe a continuous live-cell assay for determining cell proliferation profiles using the BioSpa Automated Live Cell Imaging System, consisting of BioSpa 8 and Cytation 5.READ MORE