Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA
Poster Oct 04, 2012
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi
Recently, there has been significant interest in the use of mRNA based expression systems for gene therapy applications and for the generation and manipulation of stem cells. Several groups have shown that mRNAs are attractive vehicles for therapeutic gene expression in mammals (Kormann et. al. Nat. Biotechnol (2011) 29, 154; Kariko et. al. Molecular Therapy (2012) Epub ahead of print). Additionally, Warren et al. demonstrated highly efficient induced pluripotent stem cell (iPSCs) generation by transfection of mRNAs encoding reprogramming factors (Warren et al. Cell Stem Cell (2010) 7, 618). The authors suggested that iPSCs generated in this manner should be safer than iPSCs derived by plasmid transfection or viral transduction as there is no risk of insertional mutagenesis and subsequent oncogenesis.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE