Genetic integrity of rice seeds during ageing and gene bank storage by SSR analysis
Poster Jun 30, 2014
I. O. Daniel, K. A. Adeboye, O. W. Adabale, E. S. Aladele and A. Boerner
Genetic alterations during ageing of rice seeds were analyzed using SSR markers. In the first experiment, seeds of 10 accessions of rice were subjected to artificial ageing in chambers conditioned to 55°C and 72±2%RH for 72 hours. In the second experiment, seeds of 4 accessions of rice stored in the NACGRAB gene bank, Nigeria in 2011 at 5± 4°C were compared with seeds of the same accessions freshly harvested in 2013. The objectives of this project were to evaluate ageing induced genetic changes and to establish physiological thresholds for loss of genetic integrity for rice seeds. Data were collected on seed germination and a seed vigour index was estimated. Genetic changes during the ageing course were evaluated by SSR markers using a Direct™ PCR kit. Genetic drifts and distances were estimated using PAST™ software and percentage genetic integrity was estimated from the genetic distance matrices. At 72 hours of ageing, seed germination percentage declined to 54.2% and vigour index 1.38 coinciding with the lowest estimate of genetic integrity (99.5%). Probability of genetic drift during ageing as revealed by primer RG178 was significant (P<0.005) for CG-14, IR-64 NERICA-1 and NERICA-L-34 and WITA-4. Differences in genetic distances were highest and estimate of genetic integrity was least in NERICA-1. Germination of seeds in gene bank after one and two years storage was above 99% and there were no significant genetic drifts for all accessions. The implications of result for gene bank activities will be discussed.
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial AutosamplerPoster
Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.
Assessment of Oral LISPRO Treatment in Ameliorating Amyloid and Tau Pathology in Transgenic Alzheimer’s Mice ModelPoster
Ionic co-crystals of lithium salicylate with organic proline (LISPRO) showed better safety and pharmacokinetic profile of lithium in plasma and brain of wild-type and transgenic Alzheimer mice model compared to lithium salts.READ MORE
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)Poster
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.READ MORE
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International Conference on Genomics and Pharmacogenomics
Jul 18 - Jul 19, 2018