Genomewide Profiling After Gene Knockdown by RNAi

RNAi allows targeted post transcriptional gene knockdown by the introduction of double-stranded RNA into a cell. In this study, siRNA-mediated RNAi has been combined with genomewide expression profiling using high-density oligonucleotide probearrays. This combination of technologies provides a vast amount of information about gene function and allows optimization of RNAi experiments by monitoring the effects of siRNA transfection on every gene of the genome. CDC2 was efficiently knocked down using siRNA designed by QIAGEN. After knockdown, expression profiling was performed using Affymetrix® GeneChip® arrays.