Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions

Poster   Jul 08, 2014

Genotyping-by-Sequencing of a set of diverse spring barley (<i>Hordeum vulgare</i>) accessions
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Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)


This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

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Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?


Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.


The Role of K13 in Artemisinin Resistance


Plasmodium falciparum is evolving resistance to Artemisinin Combination Therapy. The gene with the strongest association with resistance is K13. K13 is an ortholog of the well characterized transcriptional regulator Keap1. In this work we transcriptionally characterized a mutant with a transposon inserted in the K13 promoter region which results in dysregulation of K13 at 2 points of the intraerythrocytic cycle of the life-cycle to identify the processes regulated by K13.


Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2)


PfABCk2 gene is ligated into pET21a+ vector with His-tag at C-terminus and transformed into BL-21 (DE3) competent cells that are verified through Miniprep and DNA sequencing. Furthermore, this gene construct is utilized to heterologous express this protein with IPTG and afterwards purified the recombinant protein kinase using nickel affinity chromatography as shown on 10% SDS-PAGE with the expected 36 kDa protein band.



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