Gold Coated Magnetic Nanoparticles for Specific Separation and Detection of Oligonucleotide Sequences and Proteins
Poster Jan 16, 2007
Pedro Quaresma, Ricardo Franco, Pedro Baptista, Patricia Carvalho and Eulalia Pereira
Monodisperse magnetic nanoparticles were produced and subsequently coated with a gold layer by the reduction at surface of a gold precursor in order to develop an integrated detection and “gene fishing” system. Functionalization of the nanoparticles could allow the application of this system to a variety of biomolecules.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE