GPVI-RBL-2H3-NFAT-Re Luciferase Cell Line: a New Cell Based System for Studying Collagen Receptor Activation
Poster Jun 08, 2005
Marcella De Silvestris, Claudia Caserini, Maria Grazia Giribaldi, Silvia Bovolenta and Lia Scarabottolo
The response of platelets to vessel wall injury is a primary event in arterial thrombosis. Platelets respond to vessel wall injury via activation of the Collagen surface receptor Glycoprotein VI (GPVI), which accelerates the thrombotic response through release of granule contents and activation of platelet integrins.
GPVI, which represents a relevant antithrombotic pharmacological target, is a platelet membrane protein constitutively associated with the Fc Receptor y chain (FcR y). Upon activation by the specifi c ligand Collagen, GPVI induces a kinase cascade, resulting in the stimulation of PLC-y2 which fi nally leads to cytosolic Ca++ increase (Figure 1).
Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAsPoster
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
Exploiting Polypharmacology in Precision Oncology: Identification of Differential Kinase Off-targets Among Clinical PARP InhibitorsPoster
Can we use computational methods to identify previously unknown off-targets of PARP inhibitors that can explain their observed differences?READ MORE
Bioluminescent Assay for GTPases Allows Measurement of GTPase, GAP and GEF ActivitiesPoster
We have developed a homogenous bioluminescent assay (GTPase-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.READ MORE