High Content Analysis of Neural Stem Cell Expansion and Differentiation
Poster Dec 19, 2011
Oksana Sirenko, Allan C. Powe, Steven L. Stice, Karen Cook, Nick Callamaras, Jayne Hesley, Xin Jiang and Evan F. Cromwell
High content imaging methods that allow automatic evaluation of proliferation and
differentiation of hESC derived neuronal progenitors have been described.
The following effects of positive and negative factors on neuronal development have been demonstrated.
- EPO promotes differentiation of human neural progenitors toward neurons.
- No inducer SNAP, Antimycin A and staurosporine inhibit neuronal development.
These methods can be used to automate assays for:
- Testing biologics or chemical compounds on neuronal development
- Screening and validation of drug candidates
- Evaluating potential neurotoxic effects of different agents
Stem cell derived neurons and high content imaging is known to provide a powerful platform for neurobiology research and drug screening.
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluationPoster
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.READ MORE
A luminescent solar concentrator-based photomicroreactor for energy efficient continuous-flow photocatalysisPoster
The Luminescent Solar ConcentratorPhotomicroreactor
(LSC-PM) is an innovative device
for solar-powered continuous-flow photochemistry.