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High Throughput Profiling of siRNA and miRNA Libraries using Microplate Cytometry


In this study, we have used microplate cytometry for the phenotypic profiling of both siRNA and miRNA libraries. Data are presented for cell proliferation and cell cycle analysis using propidium iodide nuclear staining and an Acumen Explorer™ laser-scanning fluorescence microplate cytometer. Analysis of samples was rapid, taking about 10 minutes to process a 96 well microplate. This study supports the application of microplate cytometry for high throughput, high content profiling of the siRNA and miRNA libraries in adherent cell cultures.