High Throughput Profiling of siRNA and miRNA Libraries using Microplate Cytometry
Poster Oct 21, 2005
Wayne Bowen, Angie Cheng, Mike Byrom, Lily Rosa, Stephanie Brooking and Lance Ford
In this study, we have used microplate cytometry for the phenotypic profiling of both siRNA and miRNA libraries. Data are presented for cell proliferation and cell cycle analysis using propidium iodide nuclear staining and an Acumen Explorer™ laser-scanning fluorescence microplate cytometer. Analysis of samples was rapid, taking about 10 minutes to process a 96 well microplate. This study supports the application of microplate cytometry for high throughput, high content profiling of the siRNA and miRNA libraries in adherent cell cultures.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
18th International Conference on Pharmaceutics & Novel Drug Delivery Systems
May 27 - May 28, 2019