High Throughput Profiling of siRNA and miRNA Libraries using Microplate Cytometry
Poster Oct 21, 2005
Wayne Bowen, Angie Cheng, Mike Byrom, Lily Rosa, Stephanie Brooking and Lance Ford
In this study, we have used microplate cytometry for the phenotypic profiling of both siRNA and miRNA libraries. Data are presented for cell proliferation and cell cycle analysis using propidium iodide nuclear staining and an Acumen Explorer™ laser-scanning fluorescence microplate cytometer. Analysis of samples was rapid, taking about 10 minutes to process a 96 well microplate. This study supports the application of microplate cytometry for high throughput, high content profiling of the siRNA and miRNA libraries in adherent cell cultures.
For circulating cell free DNA (ccfDNA) to be used in cancer research successfully, workflow standardization is essential. Access this poster to discover tips on optimal workflow control, how to yield smaller ccfDNA fragments and the differences in quantification and qualification of ccfDNA.READ MORE
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE