High Throughput Surface Tension Measurement
Poster Jan 25, 2008
Neil L. Campbell, Jon Weaver
Understanding the surface tension properties of aqueous surfactants and polymer materials is important for the pharmaceutical, coatings, paints, inks, surfactant and household products industries. The traditional methods of measuring surface tension are tedious and time-consuming, so only a few compounds can be measured by a single user per day. With the commercial drive to reduce product time to market the following process explains the background behind the measurement of surface tension and the manor in which a flexible high throughput process for conducting this measurement was developed at the Centre for Materials Discovery (CMD) for use in the research of surfactant and pH responsive polymers.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE