High Titre BacMam viruses improve transduction efficiency of mammalian cells
Poster Oct 16, 2014
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King
Transient production of proteins in mammalian cells is fundamental to studies on gene function in health and disease. Many viral and plasmid vectors are available to enable the transfer of genes into mammalian cells, including baculovirus vectors.
Baculoviruses are insect-specific viruses that can transduce but not replicate in many mammalian cells. These BacMAM vectors utilise mammalian promoters to drive expression of target genes. One disadvantage of the current BacMAM system is that relatively high multiplicities of infection (50-200+ virus particles per cell) are often required for effective transduction.
This requires either concentration of the BacMAM virus (time-consuming/ labour intensive) or the use of chemical enhancers.
This study demonstrates that incorporation of a natural mutation in the FP25 gene into the BacMAM backbone vector can increase budded virus titres by several fold. Thus transduction efficacies can be improved without the need for concentration of virus or the use of chemical enhancers.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE