High-throughput imaging of cellular models using an Acumen eX3
Poster Jul 05, 2011
Paul Wylie, David Onley
The Acumen eX3 is the fastest imaging system available, collecting and simultaneously analysing over 40 images/second, covering the entire well, without the trade off of having to use lower resolution. Acumen is well established for cell-based high-content screening, but researchers have recently applied its large field of view to rapidly analyse complex cellular or animal models, such as angiogenic tube formation, C. elegans or drosophila larvae.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE