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HIV POC testing by ssDNA coupled with NALF
Poster

HIV POC testing by ssDNA coupled with NALF

HIV POC testing by ssDNA coupled with NALF
Poster

HIV POC testing by ssDNA coupled with NALF

A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.
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