Hypothesis of an Existence of a Reverse Pathway (Rp) which Passes Genetic Information from Polypeptide Antigens to Ig Genes in B-Lymphocytes
Poster Sep 23, 2013
Victor J. Alexander
Current Clonal Selection Theory fails to adequately explain the presence of a “hyper mutation” particularly in 3 CDR sections of a V part of Ig genes, which are responsible for recognition and binding of antigens with a high affinity, the degree of wastefulness of random rearrangements of Ig genes in B- lymphocytes and a standard formation of four special recognition palindromic sequences after the1st V-D-J rearrangement.
The precision of the process allows for a hypothesis of an existence of a Reverse Pathway(RP) in B-lymphocytes which passes the exact genetic information from polypeptide antigens back to DNA, permitting for versatility and quick reaction in B-lymphocytes in response to foreign antigens.
The discovery of such a pathway will, undoubtedly, challenge the current Clonal Selection Theory of Immunology as well as the (current) Central Dogma of Molecular Biology which has established the DNA<=>RNA=>Protein pathway of genetic information. It is our hope that our pioneering Reverse Pathway Research Project will show that the genetic information can follow by both directions: DNA<=>RNA<=>Protein.
The discovery of such a reverse pathway will allow for a new, more effective and affordable treatment methods for curing many of the immune related diseases as well as cancer.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.