Hypothesis of an Existence of a Reverse Pathway (Rp) which Passes Genetic Information from Polypeptide Antigens to Ig Genes in B-Lymphocytes
Poster Sep 23, 2013
Victor J. Alexander
Current Clonal Selection Theory fails to adequately explain the presence of a “hyper mutation” particularly in 3 CDR sections of a V part of Ig genes, which are responsible for recognition and binding of antigens with a high affinity, the degree of wastefulness of random rearrangements of Ig genes in B- lymphocytes and a standard formation of four special recognition palindromic sequences after the1st V-D-J rearrangement.
The precision of the process allows for a hypothesis of an existence of a Reverse Pathway(RP) in B-lymphocytes which passes the exact genetic information from polypeptide antigens back to DNA, permitting for versatility and quick reaction in B-lymphocytes in response to foreign antigens.
The discovery of such a pathway will, undoubtedly, challenge the current Clonal Selection Theory of Immunology as well as the (current) Central Dogma of Molecular Biology which has established the DNA<=>RNA=>Protein pathway of genetic information. It is our hope that our pioneering Reverse Pathway Research Project will show that the genetic information can follow by both directions: DNA<=>RNA<=>Protein.
The discovery of such a reverse pathway will allow for a new, more effective and affordable treatment methods for curing many of the immune related diseases as well as cancer.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE