Identification and Characterization of Strain Specific Drug Target by Subtractive Genome Analysis of Methicillin Resistance Staphylococcus aureus
Poster Feb 20, 2013
Kiran Saeed and Reaz Uddin
In the present study, the idea was to utilize the strain specific available genome or proteome by using ‘subtractive genomics approach’ which helped in the identification and characterization of strain specific drug targets along with comparative analysis within two of the Methicillin resistance Staphylococcus aureus (MRSA) strains i.e., MRSA398 and MRSA252.The protocol involved various bioinformatics’ tools and databases like similarity search between pathogen and host, essentiality study using the database of essential genes, metabolic functional association study using Kyoto Encyclopedia of Genes and Genomes Database (KEGG), cellular membrane localization analysis, drug bank database.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE