Identification and classification of antifouling compounds secreted by anti MIC microorganisms. A metabolomic analysis.
Poster Mar 05, 2014
Albillos SM; Balaña-Fouce R; Montero O; Barreiro-Mendez C; Blas-Galindo E; Barros-García R; Guedella-Bustamante E; Ullán RV
BIOCORIN (New Biocoating for Corrosion Inhibition in Metal Surfaces) is a project funded by the European Union under the activities of the Seventh Framework Programme (FP 7-ENVIRONMENT). Corrosion is a global problem worldwide and the direct economic loss due to metal corrosion of infrastructures and equipments is estimated to exceed €1.32 trillion per year, which accounts for 3 to 4% of the Gross Domestic Product of industrialized countries. Among corrosion types, Microbial Induced Corrosion (MIC) caused by fouling is involved in at least 10% of the corrosion of structures, and up to 50% in the case of subterranean pipes.
BIOCORIN project, aims to develop a green alternative to the coatings and solutions used up to date for MIC corrosion control. Some of the results of this project are presented here, with the identification of several relevant antifouling compounds secreted by environmentally isolated anti-MIC strains of microorganisms via a metabolomic approach.
Metabolites were extracted from100 ml cultures incubated in Terrific Broth (TB) at 30ºC for 24 h of three anti-MIC bacterial strains grown in the presence (250 l or 2 ml) and absence of a pool of MIC extracts used as inducers originated from continental Mediterranean climate, Atlantic climate, Mediterranean climate and Oceanic climate. Each culture broth was recovered and analyzed by means of LC-MS and GC-MS (Acquity UPLC - SYNAPT G2 HDMS system QToF with ESI injection and a Thermo Scientific Focus GC - DSQ II respectively). The metabolomics analysis pointed out the differential expression of 2 compounds by GC-MS and more than 10 compounds for LC-MS (positive mode) relevant to the corrosion process.