IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION
Poster Jan 28, 2015
Taja Jesenicnik, Nataša Štajner, Jernej Jakše, Sebastijan Radišek and Branka Javornik
We screened our Verticillium spp. collection with the new taxonomic PCR-markers using simplex and multiplex PCR assays. In addition, we also evaluated an alternative ITS-sequence based approach for the identification purposes.
The new PCR-marker analysis enabled us to confirm the identity of 88 isolates from various geographic locations in Europe, North America and Japan and from various host plants, including hop, potato, tomato, cotton, olive and alfalfa. According to the new taxonomic classification, 41 isolates were identified as V. nonalfalfae, 28 as V. dahliae, 6 as V. albo-atrum, 5 as V. alfalfa, 3 as V. isaacii, 2 as V. tricorpus, 1 as V. longisporum lineage A1/D1 and 1 as V. nubilum. Identification and differentiation of V. longisporum linegaes was performed by multiplex PCR assay.
We were also able to determine all isolates by means of sequence ITS analysis, reflecting two main groups of isolates, Flavexudans and Flavnonexudans. Moreover, based on ITS sequencing analysis, we were able to obtain additional information on the subgroups within the species V. albo-atrum and V. dahliae that were not revealed by PCR-marker analysis.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
The Role of K13 in Artemisinin ResistancePoster
Plasmodium falciparum is evolving resistance to Artemisinin Combination Therapy. The gene with the strongest association with resistance is K13. K13 is an ortholog of the well characterized transcriptional regulator Keap1. In this work we transcriptionally characterized a mutant with a transposon inserted in the K13 promoter region which results in dysregulation of K13 at 2 points of the intraerythrocytic cycle of the life-cycle to identify the processes regulated by K13.READ MORE
Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2)Poster
PfABCk2 gene is ligated into pET21a+ vector with His-tag at C-terminus and transformed into BL-21 (DE3) competent cells that are verified through Miniprep and DNA sequencing. Furthermore, this gene construct is utilized to heterologous express this protein with IPTG and afterwards purified the recombinant protein kinase using nickel affinity chromatography as shown on 10% SDS-PAGE with the expected 36 kDa protein band.READ MORE