Identification and Quantitation of Mixture Components of Formulated Products by NMR
Poster Dec 17, 2019
Brent G. Pautler, Patrick Wheeler, Sergey Golotvin, Rostislav Pol, Joe DiMartino
It has become increasingly important for chemists and analysts to be able to test and determine the various components that may be present in mixtures, both qualitatively and quantitatively, to solve a wide variety of scientific problems. NMR spectroscopy has emerged as a powerful, non-destructive analytical technique that provides unique information that may not be readily accessible by other means to support the needs of various industries, including polymers, pharmaceuticals, consumer products, and nutrition, to name a few.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE