Identification of Genomic and Proteomic Signatures in Lymphomas by Inducible shRNA
Poster Feb 06, 2006
Roberto Piva, Elisa Pellegrino, Michela Mattioli, Claudia Voena, Chiara Conte, Francesco Boccalatte, Chiara Ambrogio, Andrea Manazza, Antonio Neri, Roberto Chiarle and Giorgio Inghirami
AbstractThe combination of RNA interference with gene expression profiling and proteomic analysis represent a powerful tool to characterize the signaling network mediating tumorigenesis and maintenance of ALK positive lymphomas and to individuate suitable targets for a therapeutic intervention.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE