Identification of microRNA Targets using microRNA Modulation Techniques and Gene Expression Arrays
Poster Mar 10, 2015
Emily M. Anderson, Maren Mayer, Kevin Sullivan, Barbara Robertson, Žaklina Strezoska, Annaleen Vermeulen, and Devin Leake
MicroRNAs (miRNAs) have been shown to regulate gene expression through both translational attenuation and degradation of messenger RNAs (mRNAs). Though these small noncoding RNAs are predicted to play a signicant role in development, differentiation, and disease etiology, validation of miRNA targets remains a challenge. miRNA mimics and inhibitors are valuable tools for elucidating the roles of miRNAs. Here we have transfected a human liver cell line alternately with a miRNA mimic and inhibitor to miR-122 and analyzed mRNA expression changes by whole genome microarray. By examining the overlap of messages down-regulated by miRNA mimics and up-regulated by miRNA inhibitors, we robustly identify miRNA-regulated messages, many of which have canonical seed matches and some which are not identied by standard target prediction programs.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
2nd International Conference on Pharmaceutical Research & Innovations in Pharma Industry
May 30 - May 31, 2019