Impact of the transgene MON 810 in expression of coding and non-coding RNAs in maize
Poster May 11, 2015
Rodrigues, M.1, Chegão, A. 1, Costa, A. 1, Fernandes, C.1, Quedas, F. 2, de Andrade, E.1
MON 810 transgenic maize is the unique genetically modified (GM) maize approved for cultivation within the European Union.
Prior “omics” studies on MON 810 maize in comparison with non-genetically modified near-isogenic varieties has given a broad picture on the differences between transgenic and non-transgenic maize. microRNAs are small non-coding RNAs known to regulate gene expression of several genes. Here, in these study we aimed to find differences in expression of coding and non-coding genes caused by the presence of the transgene. For that, we have performed microarrays of mRNA and miRNAs from two pairs of maize (transgenic/ isogenic).
Interestingly, our results indicate possible “side effects” associated with the transgene integration into the host genome.
Fundamentals and Comparisons for Organic Sample Extract EvaporationPoster
Sample preparation is a key step in the analysis process
Parameters for evaporation and their impact on analysis have been discussed
Improvements in matching the sample to the evaporation device characteristics can help reduce variability and improve recovery
Examples for choosing a system based on sample volume, types of analytes, sample load, and initial investment considerations
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