Impact of the transgene MON 810 in expression of coding and non-coding RNAs in maize
Poster May 11, 2015
Rodrigues, M.1, Chegão, A. 1, Costa, A. 1, Fernandes, C.1, Quedas, F. 2, de Andrade, E.1
MON 810 transgenic maize is the unique genetically modified (GM) maize approved for cultivation within the European Union.
Prior “omics” studies on MON 810 maize in comparison with non-genetically modified near-isogenic varieties has given a broad picture on the differences between transgenic and non-transgenic maize. microRNAs are small non-coding RNAs known to regulate gene expression of several genes. Here, in these study we aimed to find differences in expression of coding and non-coding genes caused by the presence of the transgene. For that, we have performed microarrays of mRNA and miRNAs from two pairs of maize (transgenic/ isogenic).
Interestingly, our results indicate possible “side effects” associated with the transgene integration into the host genome.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
5th International Congress on Epigenetics & Chromatin
Aug 22 - Aug 23, 2019
International Conference on e-Health and Healthcare Innovations
May 08 - May 09, 2019