Improving the efficiency of 384 "mini-tube" technology using mosquito nanlolitre pipetting
Poster Feb 28, 2008
Joby Jenkins, Wayne Bowen, Rob Lewis & Chloe Milburn
384-well plate “mini-tube” consumables have been developed by automation companies such as REMP and The Automation Partnership (TAP) to hold compound samples divided into single-use storage tubes. Each tube holds 40 -75 µL, and is filled, sealed, stored and then thawed just once before use. However, when accessed by conventional pipetting technology, the tube’s practical working volume is as little as 20 µL, which means high compound wastage. The seal must also be pierced prior to pipetting to provide access to the sample.
TTP LabTech’s mosquito® liquid handler uses positive displacement pipetting which makes it uniquely suitable for this niche application. The disposable micropipettes can pierce foil or plastic seals, so there is no need for standalone piercing equipment. The micropipettes can reach to the bottom of 384 mini-tubes, leaving minimal dead volume. Mosquito is also able to dispense nanolitre quantities in order to create multiple miniaturised confirmation assays or serial dilutions from hit samples.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
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