Incorporation of Nanolitre Pipetting Technology into Medium and High Throughput SNP Genotyping Platforms at KBiosciences
Poster Nov 03, 2005
Phil Robinson, Aoife Gallagher
AbstractAn area of great interest in drug discovery at present is the process of genotyping in order to better understand how single nucleotide polymorphisms(SNPs) are associated with various disease states.
Companies are actively pursuing genotyping processes in medium and highthroughput fashion that will enable a large number of samples to be monitored in a short time frame and with minimal labour intensity. The genotyping process can be costly to run at all levels, especially in the consumption of expensive reagents such as PCR mixes.
spot-on™ nanolitre technology from Deerac Fluidics™ has been incorporated by UK genotyping services company KBiosciences into their high throughput system, which has enabled cost savings of up to 20 fold on previous techniques used.
This poster will describe the current process employed by KBiosciences. The vital role spot-on™ technology, incorporated into the Equator™ benchtop robotic liquid handling instrument, has played a in improving performance and reducing costs are highlighted. This has enabled KBiosciences to become cost competitive in the marketplace in the provision of their service.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.