Incorporation of Nanolitre Pipetting Technology into Medium and High Throughput SNP Genotyping Platforms at KBiosciences
Poster Nov 03, 2005
Phil Robinson, Aoife Gallagher
AbstractAn area of great interest in drug discovery at present is the process of genotyping in order to better understand how single nucleotide polymorphisms(SNPs) are associated with various disease states.
Companies are actively pursuing genotyping processes in medium and highthroughput fashion that will enable a large number of samples to be monitored in a short time frame and with minimal labour intensity. The genotyping process can be costly to run at all levels, especially in the consumption of expensive reagents such as PCR mixes.
spot-on™ nanolitre technology from Deerac Fluidics™ has been incorporated by UK genotyping services company KBiosciences into their high throughput system, which has enabled cost savings of up to 20 fold on previous techniques used.
This poster will describe the current process employed by KBiosciences. The vital role spot-on™ technology, incorporated into the Equator™ benchtop robotic liquid handling instrument, has played a in improving performance and reducing costs are highlighted. This has enabled KBiosciences to become cost competitive in the marketplace in the provision of their service.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Novel Role of the Innate Immune DNA Sensor IFI16 (Interferon Gamma Inducible Protein 16) as a Major Epigenetic Modulator During KSHV Infection and Lytic ReactivationPoster
Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE
A new method for generating arrayed RNAi screening tools for any organismPoster
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE
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2nd Annual Artificial Intelligence in Drug Development Congress
Sep 20 - Sep 21, 2018