Innovative Technology that Enables RNAi in Difficult to Transfect Cells
Poster Apr 27, 2015
Christina Yamada, Kathryn Robinson, Allison St. Amand, Zaklina Strezoska, Greg Wardle, Anastasia Khvorova, Devin Leake
Delivery remains one of the last barriers for applying RNA interference (RNAi) in clinically relevant cell lines. Investigations at Dharmacon have led to the development of an innovative molecule for delivery in a wide variety of cell types. These modified siRNAs have been found to effectively silence target genes in cell types that are typically difficult to transfect using standard delivery methods. We present data for multiple cell types including SH-SY5Y (neuroblastoma), Jurkat (T-cells), and primary neurons. This technology, Dharmacon™ Accell™ siRNA reagents, allows for functional genomic studies in pertinent cell types. Moreover, in some instances, cells can be continuously dosed with these siRNAs, thus enabling knockdown of any target gene of interest for extended durations.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE