In-plane detection of fluorescence signals in microfludic lab-on-chip flow cytyometry
Poster Jun 03, 2010
Several means of extracting fluorescence signals from flowing cell suspensions in a single plane are examined. Simple microfluidic flow cytometer structures incorporating lateral hydrodynamic focusing were molded in PDMS. Several geometries for embedding optical fibers and custom molded waveguides into the same structure were compared. Improvement in light yield is examined with molded cylindrical lenses and by using channels filled with high refractive index polymers as waveguides.
The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.READ MORE