Integrated platform including automated bligh and dyer extraction and dual-column
Poster Jun 18, 2014
Emmanuel Varesio, Guenter Boehm, Sandra Jahn, Renzo Picenoni, Gérard Hopfgartner
Sample preparation workflows for metabolomic studies of tissues or cells require most of the time a Bligh and Dyer extraction or one of its variant (e.g. the Folch extraction). This step is cumbersome and generally performed manually in order to separate the aqueous fraction containing polar endogenous metabolites from the
organic fraction containing apolar compounds like lipids. Proteins remain at the interface of the two solvents.
Here we propose to integrate an automated Bligh and Dyer extraction on a robotic system including a dual-column UHPLC-MS/MS platform for the metabolomic analysis of tissues or cells. The aqueous fraction is split and analysed sequentially at two different mobile phase pH values, whereas the lipidic fraction is analysed alternately with an extended gradient.
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Pyrolysis-GC/MS is an effective tool in introducing and analyzing polymers. But since the polymer must be broken down to volatile segments in order to be sent to the GC, identification by the Mass Spec does not look at the original polymer, but only the fragments. This poster will look at a unique library designed to work with the existing NIST library, however contains averaged spectra of pyrolyzed polymers so it can be used to directly identify the original polymer sample.READ MORE
Application of Solid Phase Micro Extraction (SPME) and On‐Fiber Derivatization to Detect Precursors of Biomolecules in the study of Proto‐metabolismPoster
In the studies of origins of life, plausible synthetic reaction pathways to form biological relevant molecules, or building blocks of proto‐bilayer lipids, protein, RNA, and DNA in the primitive environment have been investigated intensively.READ MORE
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial AutosamplerPoster
Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.READ MORE