Isolation, Identification, and Determination of Designer Anabolic Steroids Commonly Found in Dietary Supplements
Poster Mar 24, 2014
Sarah E. Voelker, M.S.; Travis M. Falconer, Ph.D.; Jonathan J. Litzau; Mary B. Jones; Lisa M. Lorenz
The marketing of “pro-hormone” dietary supplements for athletes seeking to
increase muscle mass, strength, endurance, and recovery time has increased dramatically in recent years. Stricter drug testing regulations have prompted a few corrupt supplement manufacturers to use chemically-modified structures of existing anabolic steroids, apparently in an attempt to evade detection. These “designer” steroids are expected to convert to active hormones in the body, producing the desired effect. Although little is known about the pharmacological effects of these compounds, it is likely that they, like their banned counterparts, may cause serious long-term adverse health consequences. Several steroid-like compounds in various dietary supplements have recently been detected in our laboratory. These compounds could not be readily identified due to the lack of library reference spectra or commercially available standards. The general analytical approach to these emerging compounds will be presented, including analysis by GC-MS, LCMS, and/or HPLC-UV. Analytical scale high performance liquid chromatography with fraction collection was used to isolate and collect portions of several new designer steroids observed in samples received. The isolated compounds were then characterized by Nuclear Magnetic Resonance (NMR) spectroscopy and high resolution accurate mass-mass spectrometry (HRAM-MS) to elucidate their structure. Quantitative analysis of these emerging substances in some representative dietary supplements was achieved using HPLC-UV comparison to structurally related reference standards.
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial AutosamplerPoster
Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.
Assessment of Oral LISPRO Treatment in Ameliorating Amyloid and Tau Pathology in Transgenic Alzheimer’s Mice ModelPoster
Ionic co-crystals of lithium salicylate with organic proline (LISPRO) showed better safety and pharmacokinetic profile of lithium in plasma and brain of wild-type and transgenic Alzheimer mice model compared to lithium salts.READ MORE
Analysis of Urine SRMs Using Solid-Phase Micro Extraction, Dynamic Headspace, and Liquid Injection with Comprehensive GCxGC High Res TOFMSPoster
We used modern-day, high-resolution time-of-flight mass spectrometry (HRT) and powerful processing software to quickly and confidently identify compounds in urine.READ MORE
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