Kinetic Analysis: Spotting the Weakest Binders
Designing and performing assays that accurately measure small molecule binding kinetics is an important part of the drug discovery process. It allows researchers to predict the efficacy of potential novel therapeutics and make quick decisions about weak binders.
While traditional surface plasmon resonance (SPR) approaches remain widely used for such analyses, they can not always resolve the weakest interactions.
This poster highlights how grating-coupled interferometry (GCI) can be used to resolve all interactions including the weakest ones, even when starting with just a crude reaction mixture.
Download this poster to learn how to:
- Screen, rank and characterize weak binders with off-rates up to 10 s-1
- Study binding kinetics even at large analyte:ligand MW ratios (up to 1:1000)
- Experiment with crude mixtures, detergents and other additives without clogging