KingFisher - A Multifunctional Tool for Proteomics
Poster Jan 09, 2006
Mehto, M., Walker, E., Maggott, K., Richmond, T., Partanen, M., Kymalainen, V. and Lamberg, A.
IntroductionIn the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.
KingFisher technology which utilizes magnetic beads and rods offers easy to use automation for purifying several hundreds samples per day. The unique patented technology is based on concept where magnetic particles are transferred instead of liquids (Fig. 1). KingFisher magnetic particle processors offer flexible automation for customers having different needs for throughput. The processing range is from 20µl to 1000µl which enables elution in small volumes and results in highly concentrated samples. The KingFisher platform offers ultimate flexibility since it allows running different applications in one platform, for example DNA, RNA, cell and protein purification.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
15th International Conference and Exhibition on Metabolomics & Systems
Apr 29 - Apr 30, 2019