KingFisher - A Multifunctional Tool for Proteomics
Poster Sep 29, 2005
Mehto, M., Walker, E., Maggott, K., Richmond, T., Partanen, M., Kymalainen, V., Lamberg, A.
IntroductionIn the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.
KingFisher technology which utilizes magnetic beads and rods offers easy to use automation for purifying several hundreds samples per day. The unique patented technology is based on concept where magnetic particles are transferred instead of liquids (Fig. 1). KingFisher magnetic particle processors offer flexible automation for customers having different needs for throughput.
The processing range is from 20µl to 1000µl which enables elution in small volumes and results in highly concentrated samples. The KingFisher platform offers ultimate flexibility since it allows running different applications in one platform, for example DNA, RNA, cell and protein purification.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE