KingFisher - A Multifunctional Tool for Proteomics
Poster Sep 29, 2005
Mehto, M., Walker, E., Maggott, K., Richmond, T., Partanen, M., Kymalainen, V., Lamberg, A.
IntroductionIn the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.
KingFisher technology which utilizes magnetic beads and rods offers easy to use automation for purifying several hundreds samples per day. The unique patented technology is based on concept where magnetic particles are transferred instead of liquids (Fig. 1). KingFisher magnetic particle processors offer flexible automation for customers having different needs for throughput.
The processing range is from 20µl to 1000µl which enables elution in small volumes and results in highly concentrated samples. The KingFisher platform offers ultimate flexibility since it allows running different applications in one platform, for example DNA, RNA, cell and protein purification.
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Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE
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Our results indicate that CBS contains proteins that promote α-secretase like enzymatic activity. LC-MS/MS analysis in CBSF and AgBSF revealed the presence of 142 proteins of which C1 subunits and alpha-2-macroglobulin showed significantly greater levels in αCBSF compared with αAgBSF. further study showed,C1 subunits can enhance sAPPα production and Aβ reduction in cell culture conditionREAD MORE