Knockdown of p53 by Accell Self-delivering siRNA Causes Inhibition of p53-dependent DNA Damage Response in IMR-32 Neuroblastoma Cell Line and β-amyloid Toxicity in Rat Cortical Neurons
Poster Jun 12, 2015
Žaklina Strezoska, Tamara Seredenina1, Devin Leake, Annaleen Vermeulen
Neuroblastoma cell lines and primary neuronal cultures are commonly used as cellular model systems for studying cancer and neuronal development as well as being highly relevant models for the study of neurodegenerative diseases. However, most neuroblastoma cell lines and practically all primary neuronal cells suffer from low transfection efficiency due to the refractory nature of the cells to lipid-based transfection reagents. As such, application of siRNA for inducing RNA interference (RNAi), has limited utility in these cell types; thus limiting their usefulness for development of functional assays for screening and discovery of novel disease-relevant genes.
Dharmacon™ Accell™ siRNA enables efficient delivery in a wide range of cell lines and primary cells. Accell siRNA reagents carry a novel chemical modication pattern that facilitates the delivery of siRNA without a need for transfection reagents. To demonstrate the utility of Accell siRNA reagents in neuronal cells, the effects of the down-regulation of the tumor suppressor p53 was examined. This gene plays a pivotal role in mediating DNA damage-induced apoptosis as well as conferring a protective effect from β-amyloid peptide-induced neurotoxicity. Here we describe how application of Accell siRNA enabled the development of a high content screening assay in IMR-32 neuroblastoma cells and a whole culture cell viability assay in primary rat cortical neurons. The ability to modulate gene expression in neuronal cell lines and primary neurons using Accell siRNA opens new opportunities for functional genomic siRNA screens in the eld of neuroscience.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
T-helper cell phenotype expression in cutaneous lesions of angioimmunoblastic T-cell lymphomaPoster
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE
Novel Role of the Innate Immune DNA Sensor IFI16 (Interferon Gamma Inducible Protein 16) as a Major Epigenetic Modulator During KSHV Infection and Lytic ReactivationPoster
Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE