Label-free Profiling of Ligands for Endogenous GPCRs Using a Cell-Based High Throughput Screening Technology
Poster May 19, 2006
Ye Fang, Gary Li, and Ann M. Ferrie
The resultant DMR signal offers a novel and functional optical signature for studying GPCR signaling and screening GPCR drug compounds. The theoretical considerations and assay principles are first described. The unique optical signatures for over 20 endogenous Gq, Gs and Gi-coupled receptors in A431 human epidermoid carcinoma cells are presented. As exampled in studying the signaling pathways and network interactions of endogenous GPCRs, as well as screening GPCR ligands using both kinetic and endpoint measurements, Corning® Epic™ system is an easily scaleable biosensor HTS platform for Gq, Gs and GiGPCR drug discovery and deorphanization.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE